Polarity-Sensitive Fluorescent Probefor Reflecting the Packing Degree of Bacterial Membrane Lipids
作者:
Wang, C (Wang, Chao) [1] ; Wang, JX (Wang, Jiaxin) [1] ; Xue, K (Xue, Ke) [1] ; Xiao, MH (Xiao, Minghui) [1] ; Wu, KY (Wu, Kaiyu) [1] ; Lv, S (Lv, Shuyi) [1] ; Hao, BY (Hao, Boyi) [1] ; Zhu, CL (Zhu, Chunlei) [1]
ANALYTICAL CHEMISTRY , 2022,
94(7): 3303-3312
DOI
10.1021/acs.analchem.1c05268
摘要
Themaintenanceofan intactmembranestructure isofgreat importanceforbacteria to execute various biological functions. However, chemical probesformonitoringthedynamic changesofbacterialmembranes are barely reported. Herein, we,forthefirst time, report a novelpolarity-sensitiveprobeforreflectingthepackingdegreeofbacterialmembranelipids. Specifically, we synthesize amembrane-targetingfluorescentprobe(TICT-lipid) that possesses both twist intramolecular charge transfer and aggregation-induced emission properties. TICT-lipid exhibits sensitive responses totheminute difference inthepackingdegreeofmembranelipids, facilitating rapid differentiationofGram-negative and Gram-positive bacteria. Interestingly, inthepresenceofmembrane-disrupting antibiotics,thelocalizationofTICT-lipid shifts fromtheoutermembranetothecellmembraneby outputting blue-shifted and enhanced emission, makingthemechanismofactionofantibiotics clearly visible. TICT-lipid is apolarity-sensitivefluorescentprobe, holding great promise inthestudyofmembrane-relatedbacterialprocesses and antibiotic screening.
