Sensitive fluorescence detection of saliva pepsin by a supramolecular tandem assay enables the diagnosis of gastroesophageal reflux disease

Zheng, Z (Zheng, Zhe)^{[ 1 ]} ; Geng, WC (Geng, Wen-Chao)^{[ 2 ]} ; Li, HB (Li, Hua-Bin)^{[ 2 ]} ; Guo, DS (Guo, Dong-Sheng)^{[ 2 ]}

SUPRAMOLECULAR CHEMISTRY, 2020,

DOI: 10.1080/10610278.2020.1857762

摘要

Gastroesophageal reflux disease (GERD) is a prevalent gastrointestinal disorder with substantial morbidity. However, the reliable, non-invasive, and specific diagnosis of GERD remains a challenge due to the lack of specific symptoms and imaging features. Herein, we report a supramolecular tandem assay through using a reporter pair of sulfonatocalix[4]arene and lucigenin for the sensitive and real-time detection of saliva pepsin, an ideal biomarker for GERD. Moreover, we achieve the detection of pepsin in commercial artificial saliva, demonstrating the potential of this supramolecular approach for point-of-care testing of GERD.