Encapsulated DNase improving the killing efficiency of antibiotics in staphylococcal biofilms

Liu, CH (Liu, Chenhui)^{[ 1 ]} ; Zhao, Y (Zhao, Yu)^{[ 1 ]} ; Su, WQ (Su, Wanqi)^{[ 2 ]} ; Chai, JS (Chai, Jingshan)^{[ 1 ]} ; Xu, LN (Xu, Lina)^{[ 1 ]} ; Cao, JJ (Cao, Jingjing)^{[ 1 ]} ; Liu, Y (Liu, Yang)^{[ 1 ]}

JOURNAL OF MATERIALS CHEMISTRY B, 2020, 8(20): 4395-4401

DOI: 10.1039/d0tb00441c

摘要

We developed a polymer-encapsulated DNase, n(DNase), which can efficiently accumulate in biofilm and expose the DNase to cleave the eDNA of the biofilm. CLSM and crystal violet staining results demonstrated effective biofilm disintegration (92.2%) when treated with n(DNase). This work demonstrated a general approach for coating matrix-dispersion enzymes to achieve biofilm disintegration and provided a promising strategy for treating biofilm-associated infections.