Fluorescence Monitoring of Peptide Transport Pathways into Large and Giant Vesicles by Supramolecular Host-Dye Reporter Pairs

Barba-Bon, A (Barba-Bon, Andrea)^{[ 1 ]} ; Pan, YC (Pan, Yu-Chen)^{[ 3 ]} ; Biedermann, F (Biedermann, Frank)^{[ 2 ]} ; Guo, DS (Guo, Dong-Sheng)^{[ 3 ]} ; Nau, WM (Nau, Werner M.)^{[ 1 ]} ; Hennig, A (Hennig, Andreas)^{[ 1 ]}

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2019, 141(51): 20137-20145

DOI: 10.1021/jacs.9b09563

摘要

The membrane transport mechanisms of cell-penetrating peptides (CPPs) are still controversial, and reliable assays to report on their internalization in model membranes are required. Herein, we introduce a label-free, fluorescence based method to monitor membrane transport of peptides in real time. For this purpose, a macrocyclic host and a fluorescent dye forming a host-dye reporter pair are encapsulated inside phospholipid vesicles. Internalization of peptides, which can bind to the supramolecular host, leads to displacement of the dye from the host, resulting in a fluorescence change that signals the peptide uptake and, thus, provides unambiguous evidence for their transport through the membrane. The method was successfully validated with various established CPPs, including the elusive peptide TP2, in the presence of counterion activators of CPPs, and with a calixarene-based supramolecular membrane transport system. In addition, transport experiments with encapsulated host-dye reporter pairs are not limited to large unilamellar vesicles (LUVs) but can also be used with giant unilamellar vesicles (GUVs) and fluorescence microscopy imaging.