pH-Sensitive Reversible Programmed Targeting Strategy by the Self-Assembly/Disassembly of Gold Nanoparticles

Ma, J (Ma, Jinlong)^[ 1 ] ; Hu, ZP (Hu, Zhenpeng)^[ 1 ] ; Wang, W (Wang, Wei)^[ 1 ] ; Wang, XY (Wang, Xinyu)^[ 1 ] ; Wu, Q (Wu, Qiang)^[ 1 ] ; Yuan, Z (Yuan, Zhi)^[ 1,2 ]

ACS APPLIED MATERIALS & INTERFACES, 2017, 9(20): 16768-16778

DOI: 10.1021/acsami.7b00687

 WOS:000402498600006

Abstract

A reversible programmed targeting strategy could achieve high tumor accumulation due to its long blood circulation time and high cellular internalization. Here, targeting ligand-modified poly(ethylene glycol) (PEG-ligand), dibutylamines (Bu), and pyrrolidinamines (Py) were introduced on the surface of gold nanoparticles (Au NPs) for reversible shielding/deshielding of the targeting ligands by pH responsive self-assembly. Hydrophobic interaction and steric repulsion are the main driving forces for the self-assembly/ disassembly of Au NPs. The precise self-assembly (pH >= 7.2) and disassembly (pH < ="6.8)" of au nps with different ligands could be achieved by fine-tuning the modifying molar ratio of bu and py (r-m), which followed the formula r-m="1/(-0.0013X(2)" + 0.0323x + 1), in which xis the logarithm of the partition coefficient of the targeting ligarid. the assembled/disassembled behavior of au nps at ph 7.2 and 6.8 was confirmed by transmission electron microscopy and dynamic light-scattering. enzyme-linked immunosorbent assays and cellular uptake studies showed that the ligands could be buried inside the assembly and exposed when disassembled. more importantly, this process was reversible, which provides the possibility of prolonging blood circulation by shielding ligands associated with the nps that were effused from tumor tissue.