A glucose-sensitive block glycopolymer hydrogel based on dynamic boronic ester bonds for insulin delivery

Cai, BQ (Cai, Baoqi)^[ 1 ] ; Luo, YP (Luo, Yanping)^[ 2,3 ] ; Guo, QQ (Guo, Qianqian)^[ 4 ] ; Zhang, XG (Zhang, Xinge)^[ 4 ] ; Wu, ZM (Wu, Zhongming)^[ 2,3 ]

CARBOHYDRATE RESEARCH, 2017, 445; 32-39

DOI: 10.1016/j.carres.2017.04.006

 WOS:000404501300005

Abstract

Hydrogels are good candidates to satisfy many needs for functional and tunable biomaterials. How to precisely control the gel structure and functions is crucial for the construction of sophisticated soft biomaterials comprising the hydrogels, which facilitates the impact of the surrounding environment on a unique biological function occurring. Here, glucose-responsive hydrogels comprised of 3-acrylamidophenyl boronic acid copolymerized with 2-lactobionamidoethyl methacrylate (p(APBA-b-LAMA)) were synthesized, and further evaluated as carriers for insulin delivery. The formation of (p(APBA-b-LAMA)) hydrogel was based on dynamic covalent bond using the association of boronic acid with diols. P(APBA-b-LAMA) hydrogel with the typical porous structure showed a rapid increase in equilibrium of swelling, which was up to 1856% after incubation with aqueous solution. Using insulin as a model protein therapeutic, p(APBA-b-LAMA) hydrogel exhibited high drug loading capability up to 15.6%, and also displayed glucose-dependent insulin release under physiological conditions. Additionally, the viability of NIH3T3 cells was more than 90% after treated with p(APBA-b-LAMA) hydrogel, indicating that the hydrogel had no cytotoxicity. Consequently, the novel p(APBA-b-LAMA) hydrogel has a practical application for diabetes treatment. (C) 2017 Published by Elsevier Ltd.