Single Continuous Near-Infrared Laser-Triggered
Photodynamic and Photothermal Ablation of Antibiotic-Resistant Bacteria Using
Effective Targeted Copper Sulfide Nanoclusters
Dai, XM (Dai, Xiaomei)[ 1 ] ; Zhao, Y (Zhao, Yu)[ 1 ] ; Yu, YJ (Yu, Yunjian)[ 1 ] ; Chen, XL (Chen, Xuelei)[ 1 ] ; Wei, XS (Wei, Xiaosong)[ 1 ] ; Zhang, XG(Zhang, Xinge)[ 1 ] ; Li, CX (Li, Chaoxing)[ 1 ]
ACS APPLIED
MATERIALS & INTERFACES, 2017, 9(36):
30470-30479
DOI: 10.1021/acsami.7b09638
WOS:000411043600025
Abstract
The
emergence of antibiotic-resistant bacterial strains has made conventional
antibiotic therapies less efficient. The development of a novel nanoantibiotic
approach for efficiently ablating such bacterial infections is becoming
crucial. Herein, a collection of poly(5-(2-ethyl
acrylate)-4-methylthiazole-g-butyl)/copper sulfide nanoclusters (PATA-C4@CuS)
was synthesized for efficient capture and effective ablation of
levofloxacin-resistant Gram-negative and Gram-positive bacteria upon
tissue-penetrable near-infrared (NIR) laser irradiation. In this work, we took
advantage of the excellent photothermal and photodynamic properties of copper
sulfide nanoparticles (CuSNPs) upon NIR laser irradiation and thiazole
derivative as a membrane-targeting cationic ligand toward bacteria. The
conjugated nanoclusters could anchor the bacteria to trigger the bacterial
aggregation quickly and efficiently kill them. These conjugated nanoclusters
could significantly inhibit levofloxacin-resistant Staphylococcus aureus,
Escherichia coli, Pseudomonas aeruginosa, and Bacillus amyloliquefaciens at 5.5
mu g/mL under NIR laser irradiation (980 nm, 1.5 W cm(-2), 5 min), which
suggested that the heat and reactive oxygen species (ROS) generated from the
irradiated CuSNPs attached to bacteria were effective in eliminating and
preventing the regrowth of the bacteria. Importantly, the conjugated
nanoclusters could promote healing in bacteria-infected rat wounds without
nonspecific damage to normal tissue. These findings highlight the promise of
the highly versatile multifunctional nanoantibiotics in bacterial infection.
