Ultrasensitive and specific fluorescence detection of a
cancer biomarker via nanomolar binding to a guanidinium-modified calixarene
Zheng, Z (Zheng, Zhe)[ 1 ] ; Geng, WC (Geng, Wen-Chao)[ 1 ] ; Gao, J (Gao, Jie)[ 1 ] ; Wang, YY (Wang, Yu-Ying)[ 1 ] ; Sun, HW (Sun, Hongwei)[ 1 ] ; Guo, DS(Guo, Dong-Sheng)[ 1,2 ]
CHEMICAL
SCIENCE, 2018, 9(8): 2087-2091
DOI: 10.1039/c7sc04989g
WOS:000427091500005
Abstract
We designed
a water-soluble guanidinium-modified calix[5]arene to target lysophosphatidic
acid (LPA), an ideal biomarker for early diagnosis of ovarian and other
gynecologic cancers, achieving binding on the nanomolar level. An indicator
displacement assay, coupled with differential sensing, enabled ultrasensitive
and specific detection of LPA. Moreover, we show that using a calibration line,
the LPA concentration in untreated serum can be quantified in the biologically
relevant low mu M range with a detection limit of 1.7 mu M. The reported
approach is feasible for diagnosing ovarian and other gynecologic cancers,
particularly at their early stages.