功能高分子材料教育部重点实验室

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郭东升课题组 | CHEMICAL SCIENCE

发布人:功能高分子材料教育部重点实验室    发布时间:2018/03/29   浏览次数:

Ultrasensitive and specific fluorescence detection of a cancer biomarker via nanomolar binding to a guanidinium-modified calixarene

Zheng, Z (Zheng, Zhe)1 ] Geng, WC (Geng, Wen-Chao)1 ] Gao, J (Gao, Jie)1 ] Wang, YY (Wang, Yu-Ying)1 ] Sun, HW (Sun, Hongwei)1 ] Guo, DS(Guo, Dong-Sheng)1,2 ]

CHEMICAL SCIENCE, 2018, 9(8): 2087-2091

DOI: 10.1039/c7sc04989g

  WOS:000427091500005

Abstract

We designed a water-soluble guanidinium-modified calix[5]arene to target lysophosphatidic acid (LPA), an ideal biomarker for early diagnosis of ovarian and other gynecologic cancers, achieving binding on the nanomolar level. An indicator displacement assay, coupled with differential sensing, enabled ultrasensitive and specific detection of LPA. Moreover, we show that using a calibration line, the LPA concentration in untreated serum can be quantified in the biologically relevant low mu M range with a detection limit of 1.7 mu M. The reported approach is feasible for diagnosing ovarian and other gynecologic cancers, particularly at their early stages.