Bioassemblies Fabricated by Coassembly of Protein Molecules and Monotethered Single-Chain Polymeric Nanoparticles

Liu, Q (Liu, Qi)^[ 1 ] ; Ju, YY (Ju, Yuanyuan)^[ 1 ] ; Zhao, HY (Zhao, Hanying)^[ 1,2 ]

LANGMUIR, 2018, 34(45): 13705-13712

DOI: 10.1021/acs.langmuir.8b02895

 WOS:000450695000027

Abstract

Molecular nanoparticles have been used as building blocks in the synthesis of functional materials. The grand challenges in the synthesis of the functional materials are precise control of the structures and functionalities of the materials by using nanoparticles with different architectures and properties. Monotethered single-chain polymeric nanoparticles (SCPN) are a type of nanosized asymmetric particles formed by intramolecular cross-linking of linear diblock copolymer chains. Monotethered SCPNs can be used as elemental building blocks for the fabrication of well-defined advanced structures. In this research, synthesis of biohybrid materials based on coassembly of bovine serum albumin (BSA) molecules and monotethered SCPNs is investigated. Due to the asymmetric structure of the SCPNs, positively charged SCPNs and negatively charged protein molecules coassemble into biohybrid vesicles with SCPNs on the layers and protein molecules in the walls. The self-assembled structures were analyzed by using dynamic light scattering, transmission electron microscopy, cryo-transmission electron microscopy, and atomic force microscopy. The average size of the biohybrid vesicles can be controlled by the molar ratio of SCPNs to BSA. The protein molecules in the biohybrid vesicles maintain most of the activities. This research paves a new way for the synthesis of functional biohybrid structures, and the materials can be used as protein carriers.