Degradable Protein-loaded Polymer Capsules Fabricated by Thiol-disulfide Cross-linking Reaction at Liquid-liquid Interface

Ma, XT (Ma, Xiaoteng)^{[ 1 ]} ; Han, GD (Han, Guangda)^{[ 1 ]} ; Zhao, HY (Zhao, Hanying)^{[ 1 ]}

CHINESE JOURNAL OF POLYMER SCIENCE, 2019, 37(8): 790-796

DOI: 10.1007/s10118-019-2253-9

摘要

In these years, the encapsulation of proteins for protection and delivery purpose has attracted great interest. In this research, W/O emulsion droplets were used as soft templates and bovine serum albumin (BSA) encapsulated hollow capsules were prepared by liquid-liquid interfacial thiol-disulfide exchange reaction. Block copolymer chains with pendant pyridyl disulfide groups are located at liquid-liquid interface, and upon addition of a macromolecular crosslinking agent with multiple pendant thiol groups into an emulsion, thiol-disulfide interfacial crosslinking reactions lead to the formation of BSA encapsulated hollow capsules. The cleavage of disulfides on the membranes results in the degradation of hollow structures and the release of encapsulated protein molecules. Transmission electron microscopy, scanning electron microscopy, atomic force microscopy, and confocal laser scanning microscopy were employed to characterize the hollow capsules. In comparison with native BSA, BSA molecules encapsulated in the hollow structures show higher catalytic efficiency due to higher local concentration of reactants in the structures. The membranes of the hollow capsules can efficiently protect the encapsulated BSA from hydrolysis by trypsin.