袁直课题组 | ACS BIOMATERIALS SCIENCE & ENGINEERING
发布人：功能高分子材料教育部重点实验室 发布时间：2018/11/01 浏览次数： 228 次
Construction of a Linear Cell Cross-Linker with Multivalent Glycyrrhetinic Acid Ligands for Rapid Formation of Hepatocyte Spheroids
BIOMATERIALS SCIENCE & ENGINEERING, 2018, 4(10): 3570-3577
We report herein the development of a novel cellular cross-linker further employed in the construction o(f) hepatocellular spheroids based on multivalent strategy. The linker structure consists of a glycyrrhetinic acid derivative (GA-N(CH3)(2)) modified alginate (ALG). GA-N(CH3)(2) is selected as the recognition ligand due to its high affinity toward hepatocyte, whereas ALG represents the linear backbone accounting for its good biocompatibility and flexible structure. These features endow the cellular cross-linker with the ability to flexibly interact with the cell through multiple binding sites, providing superior binding force between the cell and cross-linker, and subsequently rapidly combining multiple cells into cellular spheroids.