张新歌课题组 | BIOCONJUGATE CHEMISTRY
发布人：功能高分子材料教育部重点实验室 发布时间：2018/10/12 浏览次数： 226 次
Glycomimetic-Conjugated Photosensitizer for Specific Pseudomonas aeruginosa Recognition and Targeted Photodynamic Therapy
Zhao, Y (Zhao, Yu)[ 1 ] ; Lu, ZT (Lu, Zhentan)[ 1 ] ; Dai, XM (Dai, Xiaomei)[ 1 ] ; Wei, XS (Wei, Xiaosong)[ 1 ] ; Yu, YJ (Yu, Yunjian)[ 1 ] ; Chen, XL (Chen, Xuelei)[ 1 ]; Zhang, XG (Zhang, Xinge)[ 1 ] ; Li, CX (Li, Chaoxing)[ 1 ]
BIOCONJUGATE CHEMISTRY, 2018, 29(9): 3222-3230
Due to the rapid development of bacterial resistance, there is an urgent need to explore new antibacterial agents to substitute for traditional antibiotic therapy. Photodynamic therapy has been identified as a promising bactericidal method to conquer antibiotic-resistant pathogens. To solve the problem of photosensitizer damage to normal tissues in vivo, we developed a boron-dipyrrolemethene (BODIPY)-based glycosylated photosensitizer for ablating Pseudomonas aeruginosa (P. aeruginosa). This glycosylated photosensitizer exhibited good water solubility and generated 102 rapidly in an aqueous solution under light exposure. The photosensitizer did not cause detectable toxicity to human cells in the dark. Importantly, the photosensitizer was able to selectively attach to P. aeruginosa over normal cells, thus resulting in effective pathogen ablation by reactive oxygen species. Moreover, the photosensitizer inhibited over 90% of the biofilm formation produced by P. aeruginosa. The results indicate that the design of the macromolecular photosensitizer-induced bacterial death and inhibited biofilm formation provide a novel strategy for overcoming bacterial infection.