功能高分子材料教育部重点实验室

近期发表论文

史林启课题组 | ADVANCED FUNCTIONAL MATERIALS

发布人:功能高分子材料教育部重点实验室    发布时间:2017/12/08   浏览次数: 758 次

Eradication of Multidrug-Resistant Staphylococcal Infections by Light-Activatable Micellar Nanocarriers in a Murine Model

Liu, Y (Liu, Yong)1,2,3 ] van der Mei, HC (van der Mei, Henny C.)2,3 ] Zhao, BR (Zhao, Bingran)4,5 ] Zhai, Y (Zhai, Yan)1 ] Cheng, TJ (Cheng, Tangjian)1 ] Li, YF (Li, Yuanfeng)1 ] Zhang, ZK (Zhang, Zhenkun)1 ] Busscher, HJ (Busscher, Henk J.)2,3 ] Ren, YJ (Ren, Yijin)4,5 ] Shi, LQ (Shi, Linqi)1 ]

ADVANCED FUNCTIONAL MATERIALS, 2017, 27(44): 文献号: 1701974

DOI: 10.1002/adfm.201701974

 WOS:000416035400001

Abstract

Bacterial infections are mostly due to bacteria in their biofilm mode-of-growth, making them recalcitrant to antibiotic penetration. In addition, the number of bacterial strains intrinsically resistant to available antibiotics is alarmingly growing. This study reports that micellar nanocarriers with a poly(ethylene glycol) shell fully penetrate staphylococcal biofilms due to their biological invisibility. However, when the shell is complemented with poly(beta-amino ester), these mixed-shell micelles become positively charged in the low pH environment of a biofilm, allowing not only their penetration but also their accumulation in biofilms without being washed out, as do single-shell micelles lacking the pH-adaptive feature. Accordingly, bacterial killing of multidrug resistant staphylococcal biofilms exposed to proto-porphyrin IX-loaded mixed-shell micelles and after light-activation is superior compared with single-shell micelles. Subcutaneous infections in mice, induced with vancomycin-resistant, bioluminescent staphylococci can be eradicated by daily injection of photoactivatable protoporphyrin IX-loaded, mixed-shell micelles in the bloodstream and light-activation at the infected site. Micelles, which are not degraded by bacterial enzymes in the biofilm, are degraded in the liver and spleen and cleared from the body through the kidneys. Thus, adaptive micellar nanocarriers loaded with light-activatable antimicrobials constitute a much-needed alternative to current antibiotic therapies.